Biologists in Arizona are working to relocate the endangered Chiricahua Leopard frogs due to heavy droughts and immense livestock grazing. The Chiricahua Leopard frogs have a similar appearance to other species of leopard frogs. This makes it difficult to determine which frogs need to be relocated. Our hope is to genetically identify these frogs to help biologists relocate the correct species.

We obtained samples of an unknown frog from a project in Arizona. Our group extracted the DNA, prepared our samples for PCR reactions, then started the process of gel electrophoresis. Once we had a ladder and good band readings we prepared and sent our samples off to be sequenced by a company called GENEWIZ. All of this was done to discover what type of frog these samples were from and determine if this species was endangered.

Submitted as part of the BIO-203 Molecular Genetics Lab course.

Creator

• Kelly, Molly

• Eder, Keara

• Breeden, Kiley

Contributors

• Denton, Robert

• Arizona Game and Fish Department

• US Fish and Wildlife Service

Language

• English

Keyword

• Chiricahua Leopard frogs

• PCR reactions

• gel electrophoresis

• GENEWIZ

• Lithobates yavapainesis

• Rana yavapaiensis

Date created

• 2023

Resource type

• Poster

Rights statement

• http://rightsstatements.org/vocab/InC/1.0/